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ROM Paleohistology Workshops

Since 2013, the ROM and University of Toronto have hosted a comprehensive workshop on paleohistology research and lab techniques. The workshop is organized and run by grad students in the University of Toronto community. If interested in participating, please contact contact Dr. David Evans.

Location: Royal Ontario Museum, 100 Queen’s Park, Toronto, ON

Accommodations: Hotels close to the Royal Ontario Museum include: Howard Johnson (Avenue Rd.), Holiday Inn (Bloor St.), Comfort Inn (Charles St.), Spadina guesthouse, Madison hotel, Victoria’s Mansion

Getting Started: Meet at the staff entrance (see map), at the south end of the historic museum building 10 minutes before workshop begins each day to be signed in. The entrance is adjacent to the Museum subway station.

ROM Paleohistology Workshop Schedule 2015

Tuesday, September 1

10:00 – 10:15: Welcome and introduction to the ROM histology lab (David Evans)

10:15 – 11:00: Introduction to histology (Kirstin Brink)

-Why people use histology

-Who uses histology (useful references and books)

-Overview of techniques to be learned

-Lab rules and machine maintenance

-Specimens to be used during workshop

– Before cutting: What to do with your specimens

11:00-11:15: How to obtain specimens (Kevin Seymour/Kirstin Brink)

-Examples of paperwork

-Examples of storage

11:15-12:00: 3D Scanner and photogrammetry demo (Kentaro Chiba)

12:00–2:00: Lunch (time to go to ROM galleries)

2:00: Wire Saw Tour (Mateusz Wosik)

2:30– ~4:30

Lab work (3 groups)

1) Embedding (back of prep lab w/Kirstin)

-Complete and partial embedding with resin and technovit

2) Mounting (in histo lab w/Aaron LeBlanc and Raymond Fong)

-Mounting specimens

-Cutting on the isomet

3) Moulding and casting demos (Prep lab w/Mateusz, Shino, Ian)

-Moulding and casting small specimens

-Moulding and casting plugs in limb bones

Evening: Welcome Pub Night (Location TBA)

Wednesday, September 2

9:00–11:00: Long bone histology (Thomas Cullen)

-Bone biology and bone development

-Osteocyte Lacunar Density and LAG spacing

-Problems with histology and imaging

-Demo slides

11:00-11:15: Coffee Break

11:15-12:00: Retrocalculation and growth curve reconstruction (Kentaro Chiba)

-Ceratopsian case study

Case studies:

12:00-12:15 Mateusz Wosik: Combining size-frequency distributions and histology

12:15-12:30 David Evans: Bone microstructure of a new small theropod from Morocco

12:30–2:00 Lunch (time to go to ROM galleries)

2:00– 4:30

Lab Demos (helpers: Aaron, Kentaro, Raymond, Mateusz, Thomas, Kirstin)

-Grinding on the Hillquist (Aaron)

-Hand grinding small and large specimens (Aaron, Kentaro, Mateusz)

-Gluing/cutting large specimens (Kentaro and Mateusz)

-Thin section repair (if interested)

Lab practical

-Cutting specimens on Isomet

-Grinding specimens on the Hillquist

-Hand grinding

Evening: Free time (ROM galleries close at 5:30)

Thursday, September 3

9:00–10:45 Dental histology (Aaron)

-Tissue types, interpretations

-Demo slides

10:45–11:00: Coffee break

Case studies:

11:00–11:15: Kirstin Brink: Development of theropod dinosaur teeth

11:15-11:30: Mark MacDougall: Plicidentine in parareptiles and sphenacodontians

11:30-11:45: Raymond Fong: Coelophysis tooth histology

11:45-2:00 Lunch (Time to view ROM galleries or work on specimens)

2:00 ~4:30:

Lab demos

-Imaging (Kirstin/helpers)

Lab practicals

-Imaging (helpers)

-Hand grinding and polishing (helpers)

Evening: Wrap-up party TBA

ROM Paleohistology Workshop Schedule 2014

September 24–26, 2014

Organizers: Kirstin Brink, Aaron LeBlanc

Instructors: Kirstin Brink (UTM), Aaron LeBlanc (UTM), Kentaro Chiba (UT), Thomas Cullen(UT), Mike Burns (UA)

Wednesday, September 24th

10:00 – 10:15: Welcome and introduction to the ROM histology lab (David Evans)

10:15 – 11:00: Introduction to histology (Kirstin)

-Why people use histology

-Who uses histology (useful references and books)

-Overview of techniques to be learned

-Specimens to be used during workshop

11:00-11:15: How to obtain specimens (Kevin Seymour/Kirstin Brink)

-Examples of paperwork

-Examples of storage

11:15-12:00: Before cutting: What to do with your specimens

-Molding and casting demo in lab (Shino Sugimoto and Ian Morrison)

-3D Scanner demo (Kentaro Chiba)

12:00–2:00: Lunch

2:00– ~4:30?

Lab Demos (2 groups)

-Complete and partial embedding with resin and technovit (Kirstin)

-Isomet demo (Aaron)

Lab Practical

-Mounting specimens (Aaron)

-Cutting on the isomet (Aaron and Kirstin)

 

Evening (optional): Food and drink at restaurant/bar near ROM

Thursday, September 25th

9:00–10:30: Long Bone Histology (Michael Burns)

-Different bone types and bone development, LAGs

-Demo slides

10:30-11:00: Retrocalculation and growth curve reconstruction (Kentaro)

11:00-11:30: Osteocyte Lacunar Density and LAG spacing (Thomas Cullen)

11:30-12:30: Dental histology (Aaron)

-Tissue types, interpretations

-Demo slides

12:30–2:00 Lunch

2:00– ~4:30

Lab Demos

-Grinding on the Hillquist (Aaron)

-Hand grinding small and large specimens (Aaron and Kentaro)

-Gluing/cutting large specimens (Kentaro)

Lab practical

-Cutting specimens on Isomet (Aaron, Kentaro, Kirstin, Michael)

-Grinding specimens on the Hillquist (Aaron, Kentaro, Kirstin, Michael)

-Hand grinding (Aaron, Kentaro, Kirstin, Michael)

Evening (optional): Einstein’s or other venue

Friday, September 26th

9:45-10:00: Thomas Cullen: Problems with histology and imaging

Case Studies

10:00–10:15: Eilidh Richards: Variation in bone histology at Richards Spur

10:15–10:30: Aaron LeBlanc: Using histology to reveal ontogenetic variation in amniote dentitions

10:30–10:45: Coffee

10:45-11:00: David Evans: Bone microstructure of a new small theropod from Morocco

11:00–11:15: Kirstin Brink: The ampulla in theropod teeth

11:15-11:30: Michael Burns: Metaplasia

11:30-11:45: Mark MacDougall: Plicidentine in parareptiles and sphenacodontians

11:45–1:45 Lunch

1:45– ~4:30:

Lab demos

-Imaging (Kentaro)

-Thin section repair (Kirstin)

-Grinding large specimens (Kentaro)

Lab practicals

-Imaging (Kentaro, Kirstin)

-Hand grinding and polishing (Kentaro, Kirstin, Michael)

Evening: Wrap-up Dinner (Location TBA)

Past Participants:

2013

Brad McFeeters (Carleton University)

Evans Scott (Case Western University)

David Chapman (Cleveland Museum of Natural History)

Michael Ryan (Cleveland Museum of Natural History)

Eilidh Richards (UTM)

Diane Scott (UTM)

Mark MacDougall (UTM)

Mateusz Wosik (University of Toronto)

Kentaro Chiba (University of Toronto)

Danielle Dufalult (Royal Ontario Museum)

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